(SW5) Count, Measure, and Showcase Your Images
- Organizer:
- Richard Cole, Wadsworth Center, NYS Dept. of Health
- Instructors:
- Richard Cole, Claire Brown, Teng-Leong Chew, & Philip E. Hockberger
- The workshop will have one morning session and two afternoon sessions.
- Session 1 (Morning)
- The first session will feature presentations and hands-on:
- Image processing and analysis exercises for identifying and counting objects (e.g. noise reduction and filtering, edge detection, segmentation)
- Introduction to 3D data handling & display
- Measuring quantitative properties of objects (e.g. background and shading corrections, size, shape, intensity)
- Session 2
- 1st afternoon session – choose one, run concurrently
- 2a. Color Imaging: Getting the most information out of color images (e.g. human color perception, tricks for publishing and printing in color, differentiating control vs diseased tissues, machine learning software for color image analysis).
- 2b. Co-localization: Pixel based measurements such as Pearson’s and Mander’s co-efficients, correlation co-efficients and object based co-localization (e.g. how many early endosomes contain my protein of interest).
- Session 3
- 2nd afternoon – choose one, run concurrently
- 3a. 3D image analysis and deconvolution: Introduction to deconvolution, segmentation, counting and measuring objects in 3D data sets.
- 3b. Time-Lapse Imaging: Exercises in how to create and present time series data for supplemental movies, seminar presentations and 3D graphs for publication.
Attendees are encouraged to submit images and analysis questions/challenges ahead of time so that they can be addressed in the workshop sessions if at all possible. All presenter supplied images and class material will be available ahead of time via Google share.
Workshop Sponsors
