(SW5) Genome Engineering Using CRISPR/Cas9

Workshop Sponsors

 
 
 
 

Organizers:
CB Gurumurthy, University of Nebraska Medical Center
Eric Kmiec, Helen F. Graham Cancer Center and Research Institute, Christiana Care Health System, University of Delaware
 
Speakers:
CB Gurumurthy, University of Nebraska Medical Center
Eric Kmiec, Helen F. Graham Cancer Center and Research Institute, Christiana Care Health System, University of Delaware
 
Workshop Goals:
1. Offer participants an overall understanding of CRISPR/Cas9 technology and the applications enhanced by use of the system.
2. How to design experiments using either CRISPR alone or CRISPR with homologous donor recombination
3. How to screen for targeted mutations
4. Tools available and applied applications to facilitate gene editing
5. Provide insight on best practices of offering this service through a core facility
 
Background:
The field of genome engineering has improved drastically in the past four years. Currently the CRISPR/Cas9 system is the most popular system due to its ease of use and high levels of success rates. Scientific researchers are now equipped with a system that has the potential to make any desired change in the genome. While building a CRISPR construct is straightforward to do, many researchers are just beginning to utilize this technology in their projects and the other requirements may seem daunting. Core facilities that offer genome engineering services provide assistance in managing the overall project and offer advice on how best to design the experiment to ensure success.  
Target Audience:
Core directors, managers and technicians that desire an understanding of the CRISPR/Cas9 system in order to support genome engineering services at their organization or to collaborate on projects using these technologies.
 
Workshop Overview:
CRISPR/Cas9 basics, definitions of project types (knockout, transgenic), lingo introduced
Designing CRISPR/Cas9 target sites
Designing homologous donor recombination construct
Delivery of genome editing components (cells, mouse, zebrafish, drosophila, c. elegans, etc.)
Screening for expected mutations (PCR, T7 endonuclease I digest, Sanger sequencing, droplet digital PCR, HRMA)
 
Resources:
Internet connection capability for all attendees. Typical AV equipment such as LCD projector and screen. Attendees will be required to bring their own laptop computer.
 
Schedule:

Part 1:  CRISPR/Cas9 basics and introduction

–  Welcome and Introductions (CB Gurumurthy and Eric Kmiec)
– Genome Editing and General Introduction

Part 2:  Experimental Design

–  Designing your CRISPR experiment

–  HDR Considerations and examples of designing CRISPR mouse models

Part 3:  Attendee Participation

–  Roundtable discussions

Part 4:  Identifing CRISPR mutations

Part 5:  Panel discussion on establishing and operating a CRISPR core

 
Other Information:
Attendees will be expected to bring their own laptops. No special software is needed other than the ability to connect to the Internet.
 
 

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