(SW2) Tissue to Single Cell: Cytometry to Molecular Analysis Workflow
Workshop Sponsors
- Organizer
- E. Michael Meyer, University of Pittsburgh Cancer Institute
- Speakers
- Albert D. Donnenberg, University of Pittsburgh
- Vera S. Donnenberg, University of Pittsburgh
- Steve McClellan, Mitchell Cancer Institute
Michael W. Riggs, Cold Spring Harbor Laboratory
Steve McClellan, MT Mitchell Cancer Institute
Robert Balderas, VP Biological Sciences, BD
Don Weldon, Millipore
- Workshop goals:
- To present practical examples of cutting edge research, tutorials on techniques, and breakout sessions for in-depth discussions.
- Background:
- Tissue Cell Protein Molecule DNA
- Microscope Flow cytometry / Sorting Molecular analysis RNA / DNA
- Target Audience:
- This workshop will be of great value to researchers who are planning experiments that involve the isolation of single cells from fresh tissue as well as those planning to perform molecular analysis of single cells purified by flow cytometric cell sorting. Core resource managers will also benefit from the detailed understanding of the overall workflow and requirements of other disciplines. Individuals who are new to these techniques as well as those who wish to improve their results will gain valuable insight from this panel of speakers.
- Workshop Overview:
- This workshop would cover the complete workflow involved in successful experiments studying the genomic profiles of single cells isolated from fresh tissue. Emphasis would be placed on the hands-on techniques involved, experimental design and validation. Experts from the fields of Cytometry and Genomics will discuss their experiences developing these assays. The importance of inter-discipline collaborations will be highlighted.
- 8:00-8:15
- Introduction
Mike Meyer, UPCI Cytometry Core, University of Pittsburgh - 8:15-9:00
- Tissue Heterogeneity / Development of a Flow Cytometry Assay
Vera Donnenberg, Dept. of Cardiothoracic Surgery, University of Pittsburgh School of Medicine - 9:00-10:00
- High Content Flow Cytometry
Albert Donnenberg, Dept. of Medicine, University of Pittsburgh School of Medicine - 10:00-10:30
- Coffee Break
- 10:30-11:00
- Identifying Cellular Heterogeneity through Live Cell Biomarker Detection
Don Weldon, Millipore - 11:00-11:30
- Isolation of live cells from solid tumors based on mRNA expression
Steve McClellan, BS, MT Mitchell Cancer Institute - 11:30-12:00
- Round Table Discussion
- 12:00 to 1:00
- Lunch
- 1:00-1:30
- Optimized Cell Sorting to Enhance Molecular Analysis of Single Cells
Mike Meyer, UPCI Cytometry Core, University of Pittsburgh - 1:30-2:00
- Methods to Improve RNA quality of Sorted Cells
Scott Tighe, Advanced Genome Technologies Core University of Vermont - 2:00-2:30
- Genome-wide Copy Number Analysis of Primary Tumor Cells: Methods and Applications
Mike Riggs, Cold Spring Harbor Laboratory - 2:30-3:00
- A Simple and Rapid Workflow for High Precision, Single Cell Gene Expression Studies
Robert Balderas, VP Biological Sciences, BD - 3:00-3:30
- Coffee Break
- 3:30-4:00
- Single Cell CNV Analysis of Breast Cancer
Albert and Vera Donnenberg, University of Pittsburgh School of Medicine - 4:00-4:30
- Round Table Discussion
